Translational_Unit
virD1-5

Part:BBa_K5088703:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-08-06)


TU virD1-5 from A. tumefaciens TiBo542


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 5282
    Illegal EcoRI site found at 6329
    Illegal PstI site found at 857
    Illegal PstI site found at 3002
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 5282
    Illegal EcoRI site found at 6329
    Illegal NheI site found at 6777
    Illegal PstI site found at 857
    Illegal PstI site found at 3002
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 5282
    Illegal EcoRI site found at 6329
    Illegal BglII site found at 4979
    Illegal BglII site found at 5543
    Illegal BglII site found at 5780
    Illegal BglII site found at 7043
    Illegal BamHI site found at 1566
    Illegal XhoI site found at 6987
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 5282
    Illegal EcoRI site found at 6329
    Illegal PstI site found at 857
    Illegal PstI site found at 3002
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 5282
    Illegal EcoRI site found at 6329
    Illegal PstI site found at 857
    Illegal PstI site found at 3002
    Illegal NgoMIV site found at 1466
    Illegal NgoMIV site found at 1538
    Illegal NgoMIV site found at 4963
    Illegal NgoMIV site found at 6547
    Illegal AgeI site found at 5858
    Illegal AgeI site found at 6474
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2227
    Illegal SapI.rc site found at 5616


Design Notes

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Source

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References